Journal: Current biology : CB
Article Title: P120 Catenin Potentiates Constitutive E-cadherin Dimerization at the Plasma Membrane and Regulates trans Binding
doi: 10.1016/j.cub.2021.04.061
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-hexahistidine Aviva Systems Biology, San Diego, CA OAEA00010 Goat anti-mouse-CFL 647 IgG Santa Cruz Biotech, TX sc-362257 Anti-E-cadherin antibody DECMA-1 Sigma-Aldrich, St. Louis, MO MABT26 CFL-647 -conjugated anti-rat IgG Santa Cruz Biotech, TX sc-516660 Mouse anti-p120ctn Sigma V9131 Goat anti-mouse-IgG conjugated to Alexa Fluor 647 Invitrogen A21236 Rhodamine–phalloidin Invitrogen R415 Chemicals, peptides, and recombinant proteins BsrGI NEB Cat#R0575S EcoRI NEB Cat#R0101S Ndel NEB Cat#R0111S BamHI NEB Cat#R0136S Notl NEB Cat#R0189S Lipofectamine 2000 Invitrogen Dulbeco’s minimum Eagle’s medium (DMEM) UIUC school of chemical sciences cell facility Custom DMEM Fetal bovine serum Life Technologies, Inc Cat#11668030 Osmotic medium (10% starvation medium, 90% diH2O, 25 mM HEPES) Hristova Lab (John Hopkins University) N/A Lipofectamine 3000 Invitrogen Cat#L3000001 ProLong Gold Anti-Fade Invitrogen Cat# P10144 HEPES GIBCO Cat#15630–056 Triton X-100 Merck Cat#108643 Tween-20 Sigma Cat#P1379 T4 DNA Ligase NEB Cat#M0202S E-cad-His 6 L. Shapiro Lab (Columbia University) N/A 4% (w/v) paraformaldehyde Sigma Cat#1004960700 Critical commercial assays Alexa 647-labeled standard beads Bangs Laboratories, Fishers, IN 120-Catenin CRISPR/Cas9 Origene KN422771 Experimental models: cell lines A-431D Keith Johnson Lab (University of Nebraska N/A HEK293T American Type Culture Collection ATCC; #CRL-3216 Recombinant DNA E-cadherin-YFP/mTurq ECTM + ABD This paper N/A E-cadherin-YFP/mTurq ECTM + JMD This paper N/A E-cadherin-YFP/mTurq ECTM + JMD + ABD This paper N/A E-cadherin-YFP/mTurq 764AAA This paper N/A E-cadherin-YFP/mTurq Dileucine This paper N/A pcDNA3.1 Vector Hristova Lab (John Hopkins) https://www.snapgene.com/resources/plasmid-files/?set=image_consortium_plasmids&plasmid=pcDNA3.1(+) Actin binding region custom synthetic gene Invitrogen https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis.html pMA-T Vector custom synthetic gene Invitrogen https://www.thermofisher.com/us/en/home/life-science/cloning/gene-synthesis/geneart-gene-synthesis.html Software and algorithms FIF software Hristova Lab Stoneman, M.R., Biener, G., Ward, R.J., Pediani, J.D., Badu, D., Eis, A., Popa, I., Milligan, G., and Raicu, V. (2019).
Techniques: Recombinant, CRISPR, Plasmid Preparation, Binding Assay, Software, Fluorescence
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